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Especially for quantitative label-free proteomics studies, consistency of workflow, methodology and sample preparation in a multi-stage experimental process is key for obtaining meaningful, reproducible data. With bottom-up proteomics, this also includes consistent sample digestion prior to mass spectrometry analysis. Lebert et al. (2014) propose a universal protein standard that monitors this step, providing essential feedback about workflow optimization to researchers.1 Although other methods for monitoring Read the rest of this article
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