daily snapshot: February 28, 2016 at 04:52PM

Passport, check; courage, check; wings, check. #thisisreal
from instagram

daily snapshot: February 25, 2016 at 09:55AM

#tbt spt25feb16: Knossos
from instagram

MixGF Handles the Stats on Mixture Spectra Peptide Identifications

via Accelerating Science » Amanda Maxwell http://ift.tt/1Qc9EXW

Wang et al. (2014) present an analytical tool, MixGF, which examines the statistics of spectral probabilities for mixture spectra arising from more than one peptide and thus increases identification rates in large-scale proteomics experiments.1 Co-fragmentation of multiple peptide precursors happens frequently in large-scale mass spectrometry-based proteomics experiments, and large-scale studies are becoming the norm in Read the rest of this article

The post MixGF Handles the Stats on Mixture Spectra Peptide Identifications appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/1TFqgNv
via IFTTT

Affinity Enrichment for Mass Spectrometry-based Interactomics

via Accelerating Science » Amanda Maxwell http://ift.tt/1SMGq7v

Keilhauer et al. (2014) introduced a high-performance affinity enrichment approach for mass spectrometry (AE-MS)- based evaluation of protein-protein interactions.1 The research team contrasted the new methodology with traditional affinity purification (AP-MS), finding that it gave comparable results from a simpler workflow. In order to study the interactome, researchers must focus on the associations between proteins and Read the rest of this article

The post Affinity Enrichment for Mass Spectrometry-based Interactomics appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/1OpIzym
via IFTTT

daily snapshot: February 20, 2016 at 05:17PM

Today
from instagram

HyperLOPIT for the Mouse Stem Cell Proteome

via Accelerating Science » Amanda Maxwell http://ift.tt/1QNLeDS

When researchers want to know more about pluripotency and the push towards stem cell differentiation, they need to know more than just which genes are firing up. Christoforou et al. (2016) suggest that they also need to know what proteins are on the go, and furthermore, where they are spatially located within the cell.1   The researchers propose an upgrade of a technique Read the rest of this article

The post HyperLOPIT for the Mouse Stem Cell Proteome appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/1mLMHSP
via IFTTT

Acrylamide and Chips: LC-MS Monitoring Maillard Reaction Products in Starchy Foods

via Accelerating Science » Amanda Maxwell http://ift.tt/21aMicm

One of the major by products of the Maillard browning reaction, where reduced sugars interact with the amino acid asparagine under conditions of high heat and low moisture, is acrylamide. The IARC (International Agency for Research on Cancer) classifies this compound, a hydrophilic, polar molecule with low volatility and low molecular weight, as a potential Read the rest of this article

The post Acrylamide and Chips: LC-MS Monitoring Maillard Reaction Products in Starchy Foods appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/1R9Zt8L
via IFTTT

taking embedly for a spin ...

Man and boy Powered by RebelMouse

Clarity in Denoting Mass Spectrometric Peptide Ions

via Accelerating Science » Amanda Maxwell http://ift.tt/1Tqp1By

A recent paper published by Chu et al. (2015) gives an overview of work presented at the Mass Spectrometry and Proteomics Workshop held at the University of Hong Kong in December 2014.1 The paper contains proposals for improving the nomenclature used to describe peptide ions resulting from mass spectrometry analysis. The authors intend that the new Read the rest of this article

The post Clarity in Denoting Mass Spectrometric Peptide Ions appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/1PQizOC
via IFTTT

Patients Matter Too: Expanding the Role of Patients as Stakeholders in Biobanking

via Accelerating Science » Amanda Maxwell http://ift.tt/1POozrb

In an earlier post, we looked into the role of patients as active participants in biobank setup and management. The papers reviewed showed that, especially for disease-specific biobanks, close collaboration with donors representative of an at-risk population was key to increasing biosample acquisition. Mitchell et al. (2015) review this area further, giving evidence of disease-specific Read the rest of this article

The post Patients Matter Too: Expanding the Role of Patients as Stakeholders in Biobanking appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/1SuJN2Y
via IFTTT

Plant Antimicrobials and the Food Industry: Part 4 – Application and Regulation

via Accelerating Science » Amanda Maxwell http://ift.tt/1PYlLv0

Antimicrobials derived from plants represent an exciting new direction in food safety and product management for the food industry1. Since consumer preference is for natural products with limited perceived chemical adulteration, replacing traditional food additives with effective plant antimicrobials could win acceptance from buyers. Applications There are three main areas in which plant antimicrobials are Read the rest of this article

The post Plant Antimicrobials and the Food Industry: Part 4 – Application and Regulation appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/1PYmWug
via IFTTT

Indigestion Relief with DIGESTIF

via Accelerating Science » Amanda Maxwell http://ift.tt/1VhqzM1

Especially for quantitative label-free proteomics studies, consistency of workflow, methodology and sample preparation in a multi-stage experimental process is key for obtaining meaningful, reproducible data. With bottom-up proteomics, this also includes consistent sample digestion prior to mass spectrometry analysis. Lebert et al. (2014) propose a universal protein standard that monitors this step, providing essential feedback about workflow optimization to researchers.1  Although other methods for monitoring Read the rest of this article

The post Indigestion Relief with DIGESTIF appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/1PN5w0i
via IFTTT

on seaweed, invisible women, and Jamie Oliver

Doomsday scenario for a microbiology lab: a global agar shortage! #agarpocalypse https://t.co/6pTTrl5Vjt

— Stefanie Vogt (@StefanieVogt) November 19, 2015

#agarpocalypse

Don’t worry if this alarming piece of (non-)news slipped past your radar at the end of the year: the world is running out of seaweed!

Not just any old seaweed, but the red stuff beloved of microbiology labs.

Read more on Talk Science to Me

Proteomics Database Searching with JUMP

via Accelerating Science » Amanda Maxwell http://ift.tt/1PDjRfR

Since identifying peptides from mass spectra is an essential step in mass spectrometry (MS)-based shotgun proteomics, having efficient tools that pull meaningful and valid data out of the raw results is important for researchers. Wang et al. (2014) describe JUMP, a new algorithm developed for searching databases, which they propose as a tool complementary to Read the rest of this article

The post Proteomics Database Searching with JUMP appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/1o3GaEB
via IFTTT

Microbial Contamination in Leafy Greens: Open Fields vs Greenhouse Cultivation

via Accelerating Science » Amanda Maxwell http://ift.tt/1KClwFi

With the current attention on risk factors for foodborne illness, farms are quite rightly under scrutiny for implementing good agricultural practices (GAPs) with regard to minimizing or eradicating contamination during crop production. Holvoet et al. (2014) examined risk factors for microbial contamination in lettuce crops under open field or greenhouse growing systems1. Since fresh leafy Read the rest of this article

The post Microbial Contamination in Leafy Greens: Open Fields vs Greenhouse Cultivation appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/1QSm0Xf
via IFTTT

Enabling Proteomics Data Exchange with mzTab

via Accelerating Science » Amanda Maxwell http://ift.tt/1SZ4DpV

Proteomics researchers benefit—or suffer, depending on the point of view—from a plethora of file formats with which to store data. Some may be used because of personal preference, while others because of instrument manufacturer specifications. Although all formats store mass spectrometry data, their incompatibility may not allow exchange or comparison between studies. Furthermore, the situation Read the rest of this article

The post Enabling Proteomics Data Exchange with mzTab appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/23SzvgN
via IFTTT

daily snapshot: February 06, 2016 at 12:05PM

More in the frame: worked on this icon a little more this morning, added freezy popsicle and background. Still more to come #oils #opusdailypractice #wip #winsorandnewton
from instagram

A Proteomic Ruler That Measures Up

via Accelerating Science » Amanda Maxwell http://ift.tt/1SOxIV1

According to Wiśniewski et al. (2014), using the mass spectrometry (MS) signal generated by histones is a reliable and easily implemented first step in quantitating proteins.1  Furthermore, using the “proteomic ruler” tool developed by the authors, researchers can cut out several steps that potentially introduce error in MS-based proteomic quantitative analysis. Since its introduction into Read the rest of this article

The post A Proteomic Ruler That Measures Up appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/20Lg6vH
via IFTTT

Measuring Primary Aromatic Amine Migration into Foods by Targeted LC-HRMS

via Accelerating Science » Amanda Maxwell http://ift.tt/1PTODAa

Sanchis et al. (2015) present a sensitive and efficient method for measuring primary aromatic amine (PAA) contamination in foodstuffs using liquid chromatography coupled with high resolution mass spectrometry (LC-HRMS)1. The method the researchers propose benefits food safety monitoring by providing both targeted measurement of PAAs but also post-target screening for other common migratory contaminants such Read the rest of this article

The post Measuring Primary Aromatic Amine Migration into Foods by Targeted LC-HRMS appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/1JZOkrj
via IFTTT

Computational Proteomics: At Your Fingertips

via Accelerating Science » Amanda Maxwell http://ift.tt/1mcHH9w

The Journal of Proteomics, official publication of the European Proteomics Association (EuPA), has released a special issue on computational proteomics (2015)1 packed with original research papers on current advances in the proteomics bioinformatics world. Curated by three researchers active within the subject, Paulo Carvalho, Gabriel Padron and Yasset Perez-Riverol, the edition is a valuable resource Read the rest of this article

The post Computational Proteomics: At Your Fingertips appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/1RZZEpY
via IFTTT

Plant Antimicrobials and the Food Industry: Part 3 – Phytochemicals

via Accelerating Science » Amanda Maxwell http://ift.tt/1QE17PG

Phytochemicals are components within a plant that often impart taste or flavor. Classified as non-nutritive, these factors can also be antimicrobial—a property that has been exploited throughout history. Many cultures use herbs and spices in both cooking and medicine, as much for the pleasant sensations they impart as for their antimicrobial actions against foodborne pathogens, Read the rest of this article

The post Plant Antimicrobials and the Food Industry: Part 3 – Phytochemicals appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/1POvP5q
via IFTTT

The E. coli Acetylome: Form and Function

via Accelerating Science » Amanda Maxwell http://ift.tt/1SmSMn2

Castaño-Cerezo et al. (2014) conducted a proteomic evaluation of the Escherichia coli acetylome under four different growth conditions.1 As a post-translational modification (PTM), acetylation of lysine residues contributes to regulation of cellular activity with activation or inactivation of key proteins such as enzymes and other signaling molecules. By modifying nutrition and growth conditions in vitro, the Read the rest of this article

The post The E. coli Acetylome: Form and Function appeared first on Accelerating Science.

from Accelerating Science » Amanda Maxwell http://ift.tt/202qZqN
via IFTTT